Growth of a Pure Culture (e. Coli) and a Freshwater Microbial Community in Synthetic Media
نویسندگان
چکیده
The aim of this deliverable was to look at ways and means to study biodegradable organic carbon removal during microbial growth at low nutrient concentrations. Emphasis was placed on both carbon removal and related biomass production. Three experimental setups are described in which these basic principles were studied. These are: (1) separate batch growth of a pure culture and a natural community in complex synthetic media; (2) continuous growth of a natural community in glass bead biofilms in a lab-scale reactor with complex synthetic media: and (3) growth of a drinking water bacteria in a pilot scale activated carbon filter. Organic carbon was measured with DOC, LC-OCD and AOC analysis, while biomass was measured with cultivation independent flow cytometry and ATP analysis. The results showed that a clear and straightforward correlation could be drawn between DOC removal and biomass production, from which cellular yields can be derived.
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